BIOLOGICAL CONTROL OF TRICHOTHECIUM ROSEUM BY USING INDIGENOUS STRAINS OF TRICHODERMA HARZIANUM ISOLATED IN ALGERIA
DOI:
https://doi.org/10.4314/jfas.v12i3.21Keywords:
In vitro, antagonist, indigenous strains, T. harzianum, T. roseum.Abstract
The present study focuses on the in vitro antagonistic effect of Trichoderma harzianum against tomato cryptogrammic disease in the region of Wadi Righ; in order to introduce them into a biological control program. The fungus Trichotecium roseum was isolated from tomato plants that have fungal diseases symptoms; they were confronted directly with antagonistic strains of T. harzianum at a temperature of 26° C. The confrontation revealed that at the end of six days maximum, strains inhibited the mycelia growth of phytopathogenic species. Beyond the 6th day, T. harzianum invades the colonies of T. roseum with 57.9, 48.29 andn2.8% obtained with the strains (ST1, ST2, ST3) respectively, revealing its highly inhibitory activity. T. harzianum strains have a real antifungal potential against T. roseum and can constitute an alternative to chemicals and used as a biological control because they have succeeded in inhibiting this phytopathogenic agent growth.
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References
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[21] Elad Y, Chet I, Katan J. Trichoderma harzianum: A biocontrol agent effective Against Sclerotium rolfsii and Rhizoctonia solani. Disease control and pest management, 1979, 70 (2): 119-121, doi: 10.1094/Phyto-70-119.
[22] Moayedi G, Mostowfizadeh G R. Antagonistic activities of Trichoderma spp. On Phytophthora root rot of sugar beet. Iran Agricultural Research, 2010, 29 (1-2), 10.22099/IAR.2011.134.
[23] Hjeljord L G, Stensvand A, Tronsmo A. Antagonism of Nutrient-Activated Conidia of Trichoderma harzianum (atroviride) P1 against Botrytis cinerea. Phytopathology, 2000, 91: 1172-1180, doi: 10.1094/PHYTO.2001.91.12.1172.
[24] Gveroska B, Ziberoski J. Trichoderma harzianum as a biocontrol agent against Alternaria alternate on tobacco. Applied Technologies and Innovations, 2012, 7 (2): 67-76, doi: 10.15208/ati.2012.9.
[25] Lakhdari W, Dehliz A, Guezoul O, Acheuk F, Dekkoumi B, Benlamoudi W, M'lik R, Hammi H. Biological control of Fusarium oxysporum f. sp. radicis lycopersici by using aqueous extracts of medicinal plants of wadi righ region. Journal of plant science, 2016, 2 (1): 1-8.
[26] Yong L, Wolf G. Biological Control of Powdery Mildew by Antibiotic-producing Microorganisms Antagonistic to Erysiphe graminis. Journal of Microbiology and Biotechnology, 1995, 5 (6): 341-345.
[27] Cahagnier B, Richard-Molard D. Moisissures des aliments peu-hydratés, les moisissures. Lavoisier, 1998.
[2] Chambre d’agriculture rhone-alpe. Convertir son exploitation à l’agriculture biologique : les premières informations pratiques. CORABIO, 2015, 21 p.
[3] Pedrazzi S, Rechka Y, Pelleteret P, Chablais R, Crovadore J, Lefort F. Evaluation de biostimulants commerciaux en culture de tomate en sol. Horticulture, 2016, 48 (6): 358-364.
[4] El-Gali Z I. Trichoderma harzianum as a root treatment to control Alternaria alternate ceratoni leaf spot on Ceratonia siliqua L. Sky Journal of Agricultural Research, 2015, 4 (8): 161-166.
[5] Dal Bello G. First report of Trichothecium roseum causing postharvest fruit rot of tomato in Argentina. Australasian Plant Disease Notes, 2008, 3: 103-104, doi: 10.1007/BF03211257.
[6] Hong Y Y, Seung Y S, Chang W C, Jeum K H, Youngand S K, Seong H K. The occurrence of pink mold rot fungus Trichothecium roseum on tomatoes in Korea. Afr. J. Microbiol. Res, 2013, 7 (13): 1128-1135, doi: 10.5423/RPD.2012.18.4.396.
[7] Niu L, Yang B, Xiao-dong B, Sheng-gui Z, Hua-li X, Yong-Cai L, Wang Y, Calderón-Urrea A. Damage to Trichothecium roseum caused by sodium silicate is independent from pH.Revuecanadienne de microbiologie, 2015, 62(2): 161-172, doi: 10.1139/cjm-2015-0657.
[8] Freeman, G G, R I. Metabolic Products of Trichothecium roseum Link. Biochem. J., 1949, 45 (2): 191-199, doi: 10.1042/bj0450191.
[9] Akira I, Kazuhide K, Hiroki K, Kiyoshi T, Harukuni T, Hoyoku N. Trichothecinols A, B and C, potent anti-tumor promoting sesquiterpenoids from the fungus Trichothecium roseum. Tetrahedron Letters, 1996, 37 (51): 9219-9220.
[10] Ghosal S, Chakrabarti D K, Srivastava A K, Srivastava R S. Toxic 12,13-epoxytrichothecenes from anise fruits infected with Trichothecium roseum. J. Agric. Food Chem. 1982, 30 (1): 106-109.
[11] Lemoyne F. Les métabolites du Trichothecium roseum. 1985, 63 p.
[12] McCormick S P, Stanley A M, Stover N A, Alexander N J. Trichothecenes: From Simple to Complex Mycotoxins. Toxins (Basel), 2011, 3 (7): 802-814, doi: 10.3390/toxins3070802.
[13] Favre-Bonvin J, Jacques B, Nadia S. Biosynthesis of mycosporines: Mycosporine glutaminol in Trichothecium roseum. Phytochemistry. 1987, 26 (9): 2509-2514, doi: 10.1016/S0031-9422(00)83866-2.
[14] Özkan E, Ertan F. Production and determination of some biochemical properties of protease enzyme by Trichothecium roseum under solid state fermentation. Romanian Biotechnological letters, 2012, 17 (1): 6903-6912.
[15] Ellis M B. More Dematiaceous hyphomycetes commonwealth, Myccological Institute. 1971
[16] Barnett H L, Hunter B B. Illustrated genera of imperfect fungi. 2nd edition. Burgess Publishing Co. 1977.
[17] Benhammou N, Chet I. Parasitism of sclerotium rolfsii by Trichoderma harzianum: Ultrastructural and Cytochemical aspects of the interaction. Phytopatholoy, 1996, 86: 405-416, doi: 10.1094/Phyto-86-405.
[18] De La Cruz J J, Pintor-Toro A, Benitez T, Llobell A. Purification and caracterisation of an endo-ß-1,6-glucanase from Trichoderma harzianum that is related to its mycoparasitism. J. Bacteriol., 1995, 177: 1864-1871, doi: 10.1128/jb.177.7.1864-1871.1995.
[19] Ezziyyani M, Sánchez C P, Sid Ahmed A, Requena M E, Candela M E. Trichoderma harzianum como biofungicid apara el biocontrol de Phytophthora capsici en plantas de pimiento (Capsicum annuum L.). Anales de Biología, 2004, 26: 35-45.
[20] Berber F, Ouazzani-touhami A, Badoc A, Douira A. Antagonisme in vitro et in vivo de deux Trichoderma à l’égard de quatre espèces de Bipolaris pathogènes sur le sorgho. Bull. Soc. Pharm. Bordeaux, 2009, 148: 93-114.
[21] Elad Y, Chet I, Katan J. Trichoderma harzianum: A biocontrol agent effective Against Sclerotium rolfsii and Rhizoctonia solani. Disease control and pest management, 1979, 70 (2): 119-121, doi: 10.1094/Phyto-70-119.
[22] Moayedi G, Mostowfizadeh G R. Antagonistic activities of Trichoderma spp. On Phytophthora root rot of sugar beet. Iran Agricultural Research, 2010, 29 (1-2), 10.22099/IAR.2011.134.
[23] Hjeljord L G, Stensvand A, Tronsmo A. Antagonism of Nutrient-Activated Conidia of Trichoderma harzianum (atroviride) P1 against Botrytis cinerea. Phytopathology, 2000, 91: 1172-1180, doi: 10.1094/PHYTO.2001.91.12.1172.
[24] Gveroska B, Ziberoski J. Trichoderma harzianum as a biocontrol agent against Alternaria alternate on tobacco. Applied Technologies and Innovations, 2012, 7 (2): 67-76, doi: 10.15208/ati.2012.9.
[25] Lakhdari W, Dehliz A, Guezoul O, Acheuk F, Dekkoumi B, Benlamoudi W, M'lik R, Hammi H. Biological control of Fusarium oxysporum f. sp. radicis lycopersici by using aqueous extracts of medicinal plants of wadi righ region. Journal of plant science, 2016, 2 (1): 1-8.
[26] Yong L, Wolf G. Biological Control of Powdery Mildew by Antibiotic-producing Microorganisms Antagonistic to Erysiphe graminis. Journal of Microbiology and Biotechnology, 1995, 5 (6): 341-345.
[27] Cahagnier B, Richard-Molard D. Moisissures des aliments peu-hydratés, les moisissures. Lavoisier, 1998.
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Published
2020-08-25
How to Cite
LAKHDARI, W.; DEHLIZ, A.; BENLAMOUDI, W.; MLIK, R.; MEKHADMI, N.; HAMMI, H.; OUARGLI, D. BIOLOGICAL CONTROL OF TRICHOTHECIUM ROSEUM BY USING INDIGENOUS STRAINS OF TRICHODERMA HARZIANUM ISOLATED IN ALGERIA. Journal of Fundamental and Applied Sciences, [S. l.], v. 12, n. 3, p. 1327–1336, 2020. DOI: 10.4314/jfas.v12i3.21. Disponível em: https://mail.jfas.info/index.php/JFAS/article/view/726. Acesso em: 25 jan. 2026.
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